Using all the factors that are conducive to the preservation of strains, first freeze the microorganisms at a very low temperature (about -70 Â° C), and then use the sublimation phenomenon to remove moisture under reduced pressure (vacuum drying), so that the microorganisms are always in a low temperature, dry, lack Under the condition of oxygen, it is by far one of the most effective methods for the preservation of strains.
It is suitable for long-term preservation of strains, generally it can be stored for several years to more than ten years. But the equipment and operation are more complicated. Operation process: prepare ampoule tube â†’ prepare skimmed milk â†’ prepare strains â†’ prepare bacterial suspension â†’ sub-samples â†’ freezing â†’ vacuum drying â†’ sealing tube â†’ viability test â†’ preservation â†’ activation. The ampoule tube is soaked with 2% HCl, washed with water multiple times, and dried.
Put the label in the ampoule tube, plug the tube with cotton, sterilize at 121 â„ƒ for 30min, and set aside.
In general, bacteria require a culture of 24-48 hours; yeast needs to be cultured for 3 days; spore-forming microorganisms should be preserved; actinomycetes and filamentous fungi are cultured for 7-10 days.
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